Molecular characterization of the interaction between Tick-borne encephalitis virus NS5 protein and the Interferon α/β- and γ receptors
نویسنده
چکیده
............................................................................................................................. 3 INTRODUCTION.................................................................................................................... 3 AIM............................................................................................................................................ 6 MATERIAL AND METHODS............................................................................................... 6 PCR........................................................................................................................................ 6 TOPO®TA-cloning and restriction analysis. .................................................................... 7 Cloning into the yeast pACT2 vector. ................................................................................ 7 Yeast two-hybrid analysis.................................................................................................... 7 RESULTS.................................................................................................................................. 8 PCR........................................................................................................................................ 8 Restriction analysis of TOPO®TA-cloning. ...................................................................... 8 Cloning into yeast pACT2 vector........................................................................................ 9 Yeast two-hybrid analysis.................................................................................................. 10 DISCUSSION ......................................................................................................................... 11 ACKNOWLEDGEMENTS................................................................................................... 12 REFERENCES....................................................................................................................... 12
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TRIM79α, an interferon-stimulated gene product, restricts tick-borne encephalitis virus replication by degrading the viral RNA polymerase.
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